Epolyly®

ε-Polylysine

Model NO.: 0201
  • Introduction
  • Features
  • Applications
  • Regulations

The control of Total Plate Count (TPC) is major challenge to global food manufacturers. Polylysine has been accepted as food antimicrobial of natural origin and it is acknowledged as label-friendly. Polylysine shows high antimicrobial activity against fungi, Gram-positive and Gram-negative bacteria and exhibits good water solubility and heat stability, and does not have an unpleasant odor. However, impure Polylysine contains more or less chloride and inorganic contents, increasing the risk of endotoxin and possible interaction with other ingredients.

At Handary, Epolyly® is pure ε-Poly-L-lysine derived naturally from a submerged aerobic fermentation of Streptomyces albulus PD-1. With a high antimicrobial activity against fungi, Gram-positive and Gram-negative bacteria, Epolyly® is an ideal natural antimicrobial for extending shelf life of a variety of foods.

 

Solubility

 

Stability

Minimum inhibitory concentration of Epolyly®

 Microorganisms

MIC (ppm)

 

Molds

Aspergillus niger

250

 

Yeasts

Candida acutus

6

Phaffia rhodozyma

12

Pichia anomala

150

Pichia membranaefaciens

3

Rhodotorula lactase

25

Sporobolomyces roseus

3

Saccharomyces cerevisiae

50

Zygosaccharomyces rouxii

150

 

Gram-positive bacteria

Bacillus stearothermophilus

5

Bacillus coagulans

10

Bacillus subtilis

3

Clostridium acetobutylicum

32

Leuconostoc mesenteroides

50

Lactobacillus brevis

10

L1 plantarum

5

Micrococcus luteus

16

Staphylococcus aureus

12

Streptococcus lactis

100

 

Gram-negative bacteria

Raoultella planticola

8

Campylobacter jejuni

100

Escherichia coli

50

Pseudomonas aeruginosa

3

Salmonella typhymurium

16

DESCRIPTION

Epolyly® is pure ε-Poly-L-lysine derived naturally from a submerged aerobic fermentation of Streptomyces albulus PD-1. With a high antimicrobial activity against fungi, Gram-positive and Gram-negative bacteria, Epolyly® is an ideal natural broad-spectrum antimicrobial for extending shelf life of a variety of foods.

 

KEY BENEFITS

 Inhibition of Total Plate Count (TPC) including fungi, Gram-positive and Gram-negative bacteria;

● Increased safety by minimizing chloride and inorganic contents;

● Shelf life extension;

● Natural, consumer-friendly label.

 

APPLICATION AREAS

Bakery, Confectionery, Culinary, Dairy, Fruits & vegetables, Meat, poultry and seafood

 

DIRECTION FOR USE

In general, to deploy Epolyly® dipping solution with cold boiled water or distilled water, then direction to add into foods depending on exact application.

 

COMPOSITION

ε-Poly-l-lysine (on dry basis w/w)

 95%

Loss on drying (w/w)

 8%

Residue on ignition (w/w)

<2%

*The exact percentage variations may occur from batch to batch.

 

HEAVY METALS

Lead (mg/kg)

5

Arsenic (mg/kg)

4

 

MICROBIOLOGICAL SPECIFICATIONS

Total plate count (/g)

100

E. Coli (/g)

Absent

Salmonella (/g)

Absent

 

PHYSICAL / CHEMICAL SPECIFICATIONS

Appearance

Hygroscopic yellow powder

pH of 1% aqueous solution

6.0-7.0

Particle size

100 mesh>90%

*Appearance variations may occur from batch to batch.

 

NUTRITIONAL DATA

Approximate values for Nutrition labelling per 100g.

 

Contribution to energy level is insignificant at recommended usage levels.

Energy

   371 calories/100g

Protein

64 g/100g

Carbohydrate

27g/100g

   -Sugar

<0.5 g/100g

Fat

<0.1 g/100g

-Saturated fat

<0.1 g/100g

-Trans. fat

<0.1 g/100g

Total dietary fiber

28 g/100g

Vitamin A

<10 μg /100g

Vitamin C

7.9 mg/100g

Ash

<0.5 g/100g

 

PACKAGING

Epolyly® is available in 500 gr/bottle or 50 g/pouch with integral, tamper-proof seals. Packaging is available in other quantities.

 

Plastic bottle and pouch materials comply with Regulation (EU) No 10/2011.

 

STORAGE CONDITIONS

Conditions:

24 months, store unopened below 20°C in dry conditions, away from direct sunlight and odorous products. When opened, store at approx. 20°C in original container.

 

Shelf life:

Shelf life is 24 months when stored according to recommendations.

 

ALLERGENS

Components

Yes / No

 ● Peanut or its derivatives

No

 ● Tree nuts, macadamia nuts, pecans, pine nuts, pistachios and walnuts or their derivatives

No

 ● Sesame or its derivatives

No

 ● Eggs or its derivatives

No

 ● Fish or its derivatives

No

 ● Crustaceans and shellfish or their derivative

No

 ● Soy or its derivatives

No

 ● Wheat, triticale or their derivatives

No

 ● Mustard or its derivatives

No

 ● Milk or its derivatives (e.g. Lactose)

No

 ● Sulphites

No

 

COUNTRY OF ORIGIN

Belgium

 

CERTIFIED- STATUS

The product is certified by KOSHER, HALAL and ISO22000:2005.

 

GMO STATUS

According to regulations Ec No. 1829/2003 and 1830/2003.

 

Microorganisms used for the production of this product are not genetically modified.

 

REGULATIONS

Epolyly® ε –Poly-l-ysine has been approved as a food preservative in 4 countries, including China, Japan, South Korea and the United States. Its addition level for commonly consumed foods ranges from 0.005% to 0.05%. The regulations governing the use of Epolyly® vary considerably in the countries in which it is currently approved. Advice regarding the legal status of this product is available as appendix1.

Applications

Max. dosage

Bakery

Cake batter and cheesecakes

0.01%

Mooncake crusts

0.012%

Muffins and popovers

0.01%

Pancakes and waffles

0.025%

Pies (not fruit)

0.025%

Pie fillings

0.025%

Puddings, custards, and mousses

0.025%

Sweet pastries

0.01 - 0.025%

Confectionery

Sweet syrups and toppings

0.025%

Culinary

Boiled rice

0.02-0.05%

Cooked vegetables

0.02-0.05%

Custard cream

0.02-0.05%

Dishes

0.05%

Japanese beef steak (Sukiyaki)

0.05%

Noodles

0.05%

Potato salad

0.05%

Sliced fish (Sushi)

0.1-0.5%

Soups

0.1-0.5%

Steamed cakes

0.1-0.5%

Dairy

Cheese-based mixtures

0.025%

Cottage, ricotta, and cream chees

0.025%

Cream substitutes

0.025%

Cream

0.01%

Feta, mozzarella, and Mexican cheese

0.025%

Imitation cheese

0.025%

Processed cheese and cheese spreads

0.025%

Sour cream and dips

0.025%

Yogurt

0.01%

Fruits & vegetables

Dry beans and bean-based prepared foods

0.025%

Frozen fruit bars and sorbet

0.025%

Pickles, olives, and pickled products

0.025%

Potato- and sweet potato-based foods (excluding French fries)

0.025%

Prepared fruit and fruit mixtures

0.025%

Vegetable-based prepared foods

0.01 - 0.025%

Meat, Poultry and seafood

Fish and shellfish- based foods

0.025%

Fish and shellfish

0.025%

Meat substitutes

0.025%

APPLICATION AREAS

Select typical case studies

Epolyly™ ε –Poly-l-ysine has been approved as a food preservative in 4 countries, including China, Japan, South Korea and the United States. Its addition level for commonly consumed foods ranges from 0.1-2.5 g/kg.

Countries

Food categories

Max. dosage

China

Bakery

Bakery wares

0.15 g/kg

Meat, poultry and fishes

Cooked meat products

0.25 g/kg

Beverages

Fruit and vegetable juices

0.2 g/L

South Korea

Food preservatives  

No limit

Japan

Culinary

Boiled rice

0.02-0.05%

Cooked vegetables

0.02-0.05%

Custard cream

0.02-0.05%

Dishes

0.05%

Japanese beef steakSukiyaki

0.05%

Noodles

0.05%

Potato salad

0.05%

Sliced fish (sushi)

0.1-0.5%

Soups

0.1-0.5%

Steamed cakes

0.1-0.5%

United states

Bakery

Cake batter and cheesecakes

0.01%

Muffins and popovers

0.01%

Pancakes and waffles

0.025%

Pies (not fruit)

0.025%

Sweet pastries

0.01 - 0.025%

Pie fillings

0.025%

Puddings, custards, and mousses

0.025%

Beverage

Alcoholic beverages

0.01%

Wine

0.005%

Eggnog

0.01%

Energy, fitness, sports, and isotonic drinks (ready-to-drink)

0.025%

Gelatin, rice, and sugar cane-based beverages

0.025%

Soft drinks

0.025%

Coffee and coffee beverages

0.025%

Tea and tea beverages

0.025%

Tomato-based juices

0.025%

Nectars

0.025%

Chocolate-flavored drinks

0.01%

Fruit-flavored drinks and ades (ready to eat)

0.025%

Fruit smoothies

0.025%

Confectionary

Sweet syrups and toppings

0.025%

Culinary

Eggs

0.025%

Grain-based dishes

0.025%

Pastas

0.025%

Fat-based sauces

0.01%

Gravy

0.025%

Soups (excluding canned)

0.025%

Egg-based dishes

0.025%

Reduced-fat or fat-free margarine-like spread

0.005%

Salad dressings (regular and low-fat)

0.025%

Peanut butters and sauces

0.025%

Soy products

0.025%

Dairy

Cheese-based mixtures

0.025%

Cottage, ricotta, and cream cheese

0.025%

Feta, mozzarella, and Mexican cheese

0.025%

Processed cheese and cheese spreads

0.025%

Cream substitutes

0.025%

Imitation cheese

0.025%

Cream

0.01%

Sour cream and dips

0.025%

Yogurt

0.01%

Fruits and vegetables

Pickles, olives, and pickled products

0.025%

Dry beans and bean-based prepared foods

0.025%

Potato- and sweet potato-based foods (excluding French fries)

0.025%

Vegetable-based prepared foods

0.01 - 0.025%

Prepared fruit and fruit mixtures

0.025%

Frozen fruit bars and sorbet

0.025%

Meat, poultry and seafoods

Meat substitutes

0.025%

Fish and shellfish-based foods

0.025%

Fish and shellfish

0.025%

 

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