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- Introduction
- Applications
The control of Total Plate Count (TPC) is major challenge to global food manufacturers. Polylysine has been accepted as a food antimicrobial of natural origin and it is acknowledged as label-friendly. Polylysine shows high antimicrobial activity against fungi, Gram-positive and Gram-negative bacteria. It exhibits good water solubility and heat stability, and does not have an unpleasant odor. However, impure Polylysine contains more or less chloride and inorganic contents, increasing the risk of endotoxin and possible interaction with other ingredients.
At Handary, Epolyly® is pure ε-Poly-L-lysine derived naturally from a submerged aerobic fermentation of Streptomyces albulus PD-1. With a high antimicrobial activity against fungi, Gram-positive and Gram-negative bacteria, Epolyly® is an ideal natural antimicrobial for extending the shelf-life of a variety of foods.
Epolyly® - Effective TPC inhibitor
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Applications |
Max. Dosage |
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Bakery |
Cake batter and cheesecakes |
100 mg/kg |
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Mooncake crusts |
120 mg/kg |
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Muffins and popovers |
100 mg/kg |
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Pancakes and waffles |
250 mg/kg |
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Pies and pie fillings |
250 mg/kg |
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Puddings, custards, and mousses |
250 mg/kg |
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Sweet pastries |
250 mg/kg |
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Confectionery |
Sweet syrups and toppings |
250 mg/kg |
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Culinary |
Boiled rice |
200-500 mg/kg |
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Cooked vegetables |
500 mg/kg |
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Custard cream |
500 mg/kg |
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Dishes |
500 mg/kg |
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Japanese beef steak (Sukiyaki) |
500 mg/kg |
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Noodles |
500 mg/kg |
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Potato salad |
500 mg/kg |
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Sliced fish (Sushi) |
100-500 mg/kg |
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Soups |
100-500 mg/kg |
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Steamed cakes |
100-500 mg/kg |
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Dairy |
Cheese-based mixtures |
250 mg/kg |
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Cottage, ricotta, and cream cheese |
250 mg/kg |
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Cream and Cream substitutes |
250 mg/kg |
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Feta, mozzarella cheese |
250 mg/kg |
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Imitation cheese |
250 mg/kg |
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Processed cheese, cheese spreads and mixtures |
250 mg/kg |
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Sour cream and dips |
250 mg/kg |
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Yogurt |
100 mg/kg |
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Fruits & |
Bean-based prepared foods |
250 mg/kg |
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Frozen fruit bars and sorbet |
250 mg/kg |
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Pickles, olives, and pickled products |
250 mg/kg |
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Potato- and sweet potato-based foods |
250 mg/kg |
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Prepared fruit and fruit mixtures |
250 mg/kg |
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Vegetable-based prepared foods |
100-250 mg/kg |
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Meat, Poultry and seafood |
Fish and shellfish- based foods |
250 mg/kg |
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Fish and shellfish |
250 mg/kg |
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Meat substitutes |
250 mg/kg |
APPLICATION AREAS
Select typical case studies
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DESCRIPTION |
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Epolyly® is pure ε-Poly-L-lysine derived naturally from a submerged aerobic fermentation of Streptomyces albulus PD-1. With a high antimicrobial activity against fungi, Gram-positive and Gram-negative bacteria, Epolyly® is an ideal natural broad-spectrum antimicrobial for extending shelf life of a variety of foods. |
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KEY BENEFITS |
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● Inhibition of Total Plate Count (TPC) including fungi, Gram-positive and Gram-negative bacteria; ● Increased safety by minimizing chloride and inorganic contents; ● Shelf life extension; ● Natural, consumer-friendly label. |
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APPLICATION AREAS |
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Bakery, Confectionery, Culinary, Dairy, Fruits & vegetables, Meat, poultry and seafood |
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DIRECTION FOR USE |
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In general, to deploy Epolyly® dipping solution with cold boiled water or distilled water, then direction to add into foods depending on exact application. |
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COMPOSITION |
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ε-Poly-l-lysine (on dry basis w/w) |
≥95% |
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Loss on drying (w/w) |
≤8% |
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Residue on ignition (w/w) |
<2% |
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*The exact percentage variations may occur from batch to batch. HEAVY METALS |
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Lead (mg/kg) |
≤5 |
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Arsenic (mg/kg) |
≤4 |
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MICROBIOLOGICAL SPECIFICATIONS |
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Total plate count (/g) |
≤100 |
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E. Coli (/g) |
Absent |
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Salmonella (/g) |
Absent |
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PHYSICAL / CHEMICAL SPECIFICATIONS |
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Appearance |
Hygroscopic yellow powder |
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pH of 1% aqueous solution |
6.0-7.0 |
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Particle size |
100 mesh>90% |
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*Appearance variations may occur from batch to batch. NUTRITIONAL DATA |
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Approximate values for Nutrition labelling per 100g. Contribution to energy level is insignificant at recommended usage levels. |
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Energy |
371 calories/100g |
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Protein |
64 g/100g |
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Carbohydrate |
27g/100g |
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-Sugar |
<0.5 g/100g |
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Fat |
<0.1 g/100g |
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-Saturated fat |
<0.1 g/100g |
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-Trans. fat |
<0.1 g/100g |
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Total dietary fiber |
28 g/100g |
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Vitamin A |
<10 μg /100g |
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Vitamin C |
7.9 mg/100g |
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Ash |
<0.5 g/100g |
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PACKAGING |
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Epolyly® is available in 500 gr/bottle or 50 g/pouch with integral, tamper-proof seals. Packaging is available in other quantities. Plastic bottle and pouch materials comply with Regulation (EU) No 10/2011. STORAGE CONDITIONS |
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Conditions: 24 months, store unopened below 20°C in dry conditions, away from direct sunlight and odorous products. When opened, store at approx. 20°C in original container. Shelf life: Shelf life is 24 months when stored according to recommendations. |
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ALLERGENS |
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Components |
Yes / No |
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● Peanut or its derivatives |
No |
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● Tree nuts, macadamia nuts, pecans, pine nuts, pistachios and walnuts or their derivatives |
No |
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● Sesame or its derivatives |
No |
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● Eggs or its derivatives |
No |
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● Fish or its derivatives |
No |
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● Crustaceans and shellfish or their derivative |
No |
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● Soy or its derivatives |
No |
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● Wheat, triticale or their derivatives |
No |
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● Mustard or its derivatives |
No |
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● Milk or its derivatives (e.g. Lactose) |
No |
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● Sulphites |
No |
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COUNTRY OF ORIGIN |
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Belgium |
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CERTIFIED- STATUS |
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The product is certified by KOSHER, HALAL and ISO22000:2005. |
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GMO STATUS |
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According to regulations Ec No. 1829/2003 and 1830/2003. Microorganisms used for the production of this product are not genetically modified. |
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REGULATIONS |
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Epolyly® ε –Poly-l-ysine has been approved as a food preservative in 4 countries, including China, Japan, South Korea and the United States. Its addition level for commonly consumed foods ranges from 0.005% to 0.05%. The regulations governing the use of Epolyly® vary considerably in the countries in which it is currently approved. Advice regarding the legal status of this product is available as appendix1. |
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Epolyly™ ε –Poly-l-ysine has been approved as a food preservative in 4 countries, including China, Japan, South Korea and the United States. Its addition level for commonly consumed foods ranges from 0.1-2.5 g/kg.
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Countries |
Food categories |
Max. dosage |
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China |
Bakery |
Bakery wares |
0.15 g/kg |
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Meat, poultry and fishes |
Cooked meat products |
0.25 g/kg |
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Beverages |
Fruit and vegetable juices |
0.2 g/L |
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South Korea |
Food preservatives |
No limit |
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Japan |
Culinary |
Boiled rice |
0.02-0.05% |
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Cooked vegetables |
0.02-0.05% |
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Custard cream |
0.02-0.05% |
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Dishes |
0.05% |
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Japanese beef steak（Sukiyaki） |
0.05% |
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Noodles |
0.05% |
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Potato salad |
0.05% |
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Sliced fish (sushi) |
0.1-0.5% |
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Soups |
0.1-0.5% |
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Steamed cakes |
0.1-0.5% |
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United states |
Bakery |
Cake batter and cheesecakes |
0.01% |
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Muffins and popovers |
0.01% |
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Pancakes and waffles |
0.025% |
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Pies (not fruit) |
0.025% |
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Sweet pastries |
0.01 - 0.025% |
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Pie fillings |
0.025% |
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Puddings, custards, and mousses |
0.025% |
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Beverage |
Alcoholic beverages |
0.01% |
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Wine |
0.005% |
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Eggnog |
0.01% |
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Energy, fitness, sports, and isotonic drinks (ready-to-drink) |
0.025% |
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Gelatin, rice, and sugar cane-based beverages |
0.025% |
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Soft drinks |
0.025% |
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Coffee and coffee beverages |
0.025% |
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Tea and tea beverages |
0.025% |
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Tomato-based juices |
0.025% |
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Nectars |
0.025% |
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Chocolate-flavored drinks |
0.01% |
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Fruit-flavored drinks and ades (ready to eat) |
0.025% |
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Fruit smoothies |
0.025% |
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Confectionary |
Sweet syrups and toppings |
0.025% |
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Culinary |
Eggs |
0.025% |
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Grain-based dishes |
0.025% |
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Pastas |
0.025% |
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Fat-based sauces |
0.01% |
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Gravy |
0.025% |
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Soups (excluding canned) |
0.025% |
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Egg-based dishes |
0.025% |
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Reduced-fat or fat-free margarine-like spread |
0.005% |
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Salad dressings (regular and low-fat) |
0.025% |
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Peanut butters and sauces |
0.025% |
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Soy products |
0.025% |
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Dairy |
Cheese-based mixtures |
0.025% |
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Cottage, ricotta, and cream cheese |
0.025% |
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Feta, mozzarella, and Mexican cheese |
0.025% |
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Processed cheese and cheese spreads |
0.025% |
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Cream substitutes |
0.025% |
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Imitation cheese |
0.025% |
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Cream |
0.01% |
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Sour cream and dips |
0.025% |
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Yogurt |
0.01% |
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Fruits and vegetables |
Pickles, olives, and pickled products |
0.025% |
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Dry beans and bean-based prepared foods |
0.025% |
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Potato- and sweet potato-based foods (excluding French fries) |
0.025% |
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Vegetable-based prepared foods |
0.01 - 0.025% |
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Prepared fruit and fruit mixtures |
0.025% |
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Frozen fruit bars and sorbet |
0.025% |
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Meat, poultry and seafoods |
Meat substitutes |
0.025% |
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Fish and shellfish-based foods |
0.025% |
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Fish and shellfish |
0.025% |
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